The transition to sustainable packaging solutions is of critical importance in mitigating environmental impacts, and it is evident that consumer engagement plays a pivotal role in achieving this objective. Therefore, this study investigated consumer perception and acceptance of packaging made from bio-based or recycled materials through an integrative methodology comprising a literature review, in-person focus groups, and an online survey, as well as a final consumer test using demonstrators developed within the PRESERVE project. The findings of this study contribute to a growing body of evidence on consumer attitudes and behaviours, revealing both opportunities and challenges for advancing sustainable packaging initiatives. The findings suggest a prevailing consumer inclination towards adopting innovative, eco-friendly packaging solutions. Participants expressed strong support for reducing reliance on conventional plastics and were receptive to bio-based alternatives. However, significant barriers to widespread acceptance were also identified, including a lack of understanding of how to identify sustainable packaging and confusion regarding appropriate disposal practices. These challenges underscore the necessity for enhanced communication and clearer labelling strategies to address the existing knowledge deficit and facilitate informed consumer choices. The study's findings align with existing literature, which suggests that while consumers exhibit favourable attitudes towards sustainability, their behaviours often remain incongruent. The findings of this study, when viewed in conjunction with previous research, underscore the necessity of harmonised efforts in education, policy, and design. In order to enhance consumer acceptance and effective use of sustainable packaging, stakeholders should prioritize the development of standardised labelling systems, public awareness campaigns, and infrastructure improvements to support proper waste management.